FastQCFastQC Report
Sun 19 Mar 2023
SRR4895856_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895856_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4815840
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGTC4827600.0143.647981
TCTGTCA4827750.0143.643512
CTGTCAG1761500.0143.591223
CTGTCAT1687400.0143.445273
CTGTCAA1369150.0143.347873
TGTCAGG651650.0142.762484
TGTCATG626050.0142.631384
TGTCAGT610050.0142.595474
TGTCAGA469950.0142.375964
TGTCATA475250.0142.257724
TGTCAAT476100.0142.079354
GTCATAG174900.0141.98285
TGTCAAG492050.0141.863594
GTCATGA165550.0141.825385
GTCAGGT224900.0141.470845
GTCAGAT164850.0141.029985
GTCATGG228000.0140.842065
GTCATTG211050.0140.793145
TGTCATT569550.0140.624664
GTCAGTG212400.0140.576235
Produced by FastQC (version 0.11.5)