FastQCFastQC Report
Sun 19 Mar 2023
SRR4895856_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895856_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4815840
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG127410.26456443735672286No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG109740.2278730190371773No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG99660.2069420910993721No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC90960.1888767068673378No Hit
CCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTG88060.18285491212332636No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC87470.18162978836506197No Hit
TGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGC87090.18084072560550185No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA86610.17984401475132064No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC83490.17336539419914285No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT82400.17110202996777302No Hit
AAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATC82120.170520615302834No Hit
ATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCT80800.16777966045383566No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC80540.16723977540782087No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT78280.16254692846938437No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT77490.1609065085218778No Hit
CCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTC77010.15990979766769658No Hit
TTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATAC76670.15920379414598493No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA75890.15758413900794047No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT75600.15698195953353933No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT75580.1569404299146151No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74580.15486394896840427No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT72860.15129240174092162No Hit
TACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCT72120.1497558058407256No Hit
ACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTT71320.14809462108375693No Hit
AACCATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAA70910.1472432638958105No Hit
AAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGA70890.14720173427688626No Hit
AAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATAC70400.14618425861324297No Hit
AATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTC70390.14616349380378085No Hit
ATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATA70390.14616349380378085No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT70350.14608043456593242No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT70080.1455197847104555No Hit
CGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGACACAAA69850.145042194092827No Hit
TCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCA69760.14485531080766803No Hit
ACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGT69440.14419083690488055No Hit
GGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCT68680.14261271138576032No Hit
TGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAAC68330.14188594305458654No Hit
TGGAAGACGGCAGCCGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGC67950.1410968802950264No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT67910.141013821057178No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC67780.14074387853417059No Hit
TTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTAC67770.14072311372470847No Hit
AGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCG67170.13947722515698197No Hit
CTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAA67160.13945646034751985No Hit
TCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATG67080.139290341871823No Hit
ATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCC66620.138335160636566No Hit
TGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCAT66220.13750456825808166No Hit
GGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACG65780.13659091664174888No Hit
CTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCT65350.13569802983487825No Hit
CGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGACACAAACTC64640.13422372836306853No Hit
TTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGA64560.13405760988737167No Hit
AGCCGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGACAC63900.13268713246287253No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT63870.1326248380344862No Hit
TGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCC63570.13200189375062293No Hit
CACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTT63060.1309428884680554No Hit
TATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGAC62530.12984235356656368No Hit
TTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATG61940.1286172298082993No Hit
CTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTT61870.12847187614206454No Hit
AGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTT61860.1284511113326024No Hit
GTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATA61450.12759975414465596No Hit
TTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACA61380.12745440047842121No Hit
CTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTT61380.12745440047842121No Hit
ATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACA60540.12571015648360412No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC60050.1246926808199608No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG59660.12388285325093858No Hit
AAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGA59630.12382055882255223No Hit
AGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGA59600.12375826439416591No Hit
CATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGA59200.12292767201568158No Hit
AGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC59150.12282384796837105No Hit
TGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTG59050.12261619987374996No Hit
AAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTA58550.12157795940064453No Hit
TACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAG58420.12130801687763715No Hit
TGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTAT58400.1212664872587129No Hit
GGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACC58230.12091348549785708No Hit
AAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCGCTC58050.12053971892753912No Hit
ACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCC57760.11993753945313797No Hit
TAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATAT57630.11966759693013058No Hit
TCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACT57150.11867088607594937No Hit
AACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACT56980.11831788431509352No Hit
ACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTA56830.11800641217316189No Hit
TGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTT56620.11757035117445762No Hit
ACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACA56520.11736270307983654No Hit
TGCCTTCCTCCTTATCCTACAGTACAATCAGCAGTCTAGTTCTTTTCATT56330.11696817170005647No Hit
AGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATCAGCAGTCTAGTTC56210.11671899398651119No Hit
CTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAAT55890.1160545200837237No Hit
TTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTA55660.11557692946609521No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG55500.11524469251470149No Hit
TAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTT55040.11428951127944449No Hit
GCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGA54920.11404033356589921No Hit
GTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAA54760.11370809661450547No Hit
CTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAG54660.11350044851988438No Hit
TGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTC54610.11339662447257383No Hit
ACAACCATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGG54440.113043622711718No Hit
GGCAGCCGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGA54130.11239991361839265No Hit
TCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGC53570.11123708428851457No Hit
GCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAG53480.1110502010033556No Hit
CAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAAT53460.11100867138443136No Hit
GAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTT53300.11067643443303764No Hit
TGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAG53120.1103026678627197No Hit
AGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTAC52740.10951360510315956No Hit
CTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGC52640.10930595700853848No Hit
TGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCT52240.10847536463005414No Hit
TTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTG52230.10845459982059204No Hit
CACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTT52220.10843383501112994No Hit
TGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTA51870.10770706667995614No Hit
AAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTG51860.10768630187049404No Hit
GATGACTATCACCTCCTAAAGGACCTAGAGGAAGGCATCCAAACGCTGAT51790.10754094820425927No Hit
GAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGG51770.10749941858533506No Hit
CAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCG51730.10741635934748663No Hit
CAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCT51630.10720871125286555No Hit
TTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTT51270.10646117811222965No Hit
CTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGG51180.10627429482707067No Hit
CAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTT51030.10596282268513905No Hit
GTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACT50970.1058382338283664No Hit
GTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT50910.10571364497159375No Hit
AAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAA50760.10540217282966212No Hit
CACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTT50670.10521528954450315No Hit
CCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCT50450.10475846373633677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA50430.10471693411741254No Hit
ATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGAC50280.10440546197548091No Hit
TGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCA50100.10403169540516297No Hit
CACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGAC50070.10396940097677664No Hit
CACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACA49920.10365792883484501No Hit
TACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGT49750.10330492707398917No Hit
ATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGG49680.10315957340775442No Hit
CTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCAT49470.10272351240905014No Hit
GATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATG49310.1023912754576564No Hit
TGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCA49260.10228745141034586No Hit
GCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCA49200.10216286255357321No Hit
ACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTC49180.10212133293464899No Hit
TATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCT49070.1018929200305658No Hit
CTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTAC48940.1016229775075584No Hit
TTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACC48820.10137379979401309No Hit
CAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCC48740.10120768131831623No Hit
CTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTAT48630.10097926841423302No Hit
TACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTC48620.10095850360477092No Hit
ATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGA48620.10095850360477092No Hit
GGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCGC48400.10050167779660454No Hit
GATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGAT48350.100397853749294No Hit
ACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTAC48330.10035632413036978No Hit
CTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGT48210.10010714641682447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA510200.0141.17688
TGGTATC512650.0140.516167
GTGGTAT512600.0140.501776
AAGCAGT515200.0139.820311
CAGTGGT517350.0139.290044
AGCAGTG521250.0138.264542
GCAGTGG522800.0137.935843
GTATCAA525650.0136.931449
AGTGGTA539700.0133.433415
TCAACGC507350.028.62954910-14
ATCAACG508000.028.558910-14
CAACGCA509250.028.53121610-14
CGCAGAG510750.028.39103515-19
AGAGTAC510500.028.37673215-19
TACATGG509650.028.37602620-24
CAGAGTA512950.028.26646215-19
GTACATG512300.028.26578520-24
AGTACAT513400.028.15473720-24
GAGTACA510600.028.10607520-24
GCAGAGT516150.028.08284615-19
Produced by FastQC (version 0.11.5)