FastQCFastQC Report
Sun 19 Mar 2023
SRR4895855_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895855_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4310078
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGCA4316750.0143.776082
TATCAGC4317000.0143.767751
TCAGCAT1475500.0143.511633
TCAGCAG1592500.0143.448013
TCAGCAA1246800.0143.05833
CAGCAGT543950.0142.834764
CAGCATG533500.0142.771474
CAGCAAT429050.0142.388584
CAGCATA431050.0142.245734
CAGCATT497000.0142.00044
AGCATGG197400.0141.957035
CAGCAGG594200.0141.842744
CAGCAAG456100.0141.773764
AGCATTG187950.0141.547875
AGCAGGT201850.0141.467015
AGCAGTG182550.0141.278145
AGCATGT181750.0141.266165
AGCATAG157100.0141.249765
CAGCAGA436600.0140.981724
AGCAATG154800.0140.697275
Produced by FastQC (version 0.11.5)