FastQCFastQC Report
Sun 19 Mar 2023
SRR4895854_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895854_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9795731
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATG9819150.0143.655881
AGGATGA9840550.0143.343492
GGATGAT3634900.0142.65883
GGATGAG3320700.0142.590453
GGATGAA2916600.0142.135993
GATGATA1132700.0137.052154
GATGATG1313800.0136.458924
GATGAGG1216650.0135.986984
GATGAGA982700.0135.9774
GATGAGT1207200.0135.309954
GATGAAG1049050.0134.507784
ATGATAG398400.0134.186555
GATGAAA917900.0133.614364
ATGAGTG397450.0133.22115
ATGAGTA375550.0132.765095
GATGAAT1094350.0132.519034
ATGAGAG322650.0132.418215
ATGATTG470500.0131.987065
ATGAATG377400.0131.713655
ATGATGG473550.0129.981455
Produced by FastQC (version 0.11.5)