FastQCFastQC Report
Sun 19 Mar 2023
SRR4895854_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895854_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9795731
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG257950.26332899504896573No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG234280.23916540787002014No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT201440.20564059997155906No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG197230.20134280943402794No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT192200.1962079195518946No Hit
CCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTG191020.19500331317795477No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA190970.1949522705349912No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT189130.19307390127393248No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC188380.1923082616294792No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA187790.1917059584425093No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC185520.18938862245196403No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT183050.18686711588956456No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT181660.1854481304151778No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC180270.18402914494079103No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT179210.18294704090996372No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT176380.1800580273182267No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT175120.17877175271554518No Hit
AAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATC175020.17866966742961807No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT173480.17709755402634067No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC172390.17598482440973523No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA171400.17497418007905688No Hit
ATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCT170730.1742902086633453No Hit
CCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTC170010.17355519460467014No Hit
AATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTC168620.17213620913028338No Hit
TTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATAC165230.16867551793735452No Hit
ACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGT164640.16807321475038461No Hit
TGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAAC164590.16802217210742107No Hit
GGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACG162980.1663785990039947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC160960.1643164762282672No Hit
ATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCC158500.16180517819446041No Hit
TACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCT158000.16129475176482488No Hit
TGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCC157580.16086599356393108No Hit
CTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAA156260.1595184677896933No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGGTGTGCAACTACCGC154170.1573848853138168No Hit
ATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATA154170.1573848853138168No Hit
ACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTT153840.15704800387025736No Hit
AGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTT152850.15603735953957903No Hit
AAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATAC151860.15502671520890068No Hit
GTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATA150830.15397523676385153No Hit
TGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCAT150130.1532606397623618No Hit
CTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCT148760.15186207134516044No Hit
TTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGA148160.15124955962959782No Hit
CTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTT146710.14976932298365483No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG146170.14921806243964844No Hit
GGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACC145430.14846263132378787No Hit
TATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGAC144390.147400944350146No Hit
TACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAG143640.14663530470569272No Hit
GCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAG143020.14600237593294468No Hit
AAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGA141870.14482839514478296No Hit
TTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTAC141660.14461401604433605No Hit
GTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAA140590.14352170348491602No Hit
TCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCA140270.1431950305699493No Hit
AAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTA138900.14179646215274797No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT138690.14158208305230105No Hit
AGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC138430.1413166613088906No Hit
TGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTT137660.14053060460725186No Hit
TTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATG137650.14052039607865915No Hit
GAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTT137100.1399589270060601No Hit
ATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACA135600.13842764771715352No Hit
AGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGA135140.13795805540188885No Hit
CTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTT134830.13764159101551482No Hit
CACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTT134600.13740679485788249No Hit
TGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTG134170.13696782812839592No Hit
TGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTAT132590.13535488061074769No Hit
CTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAAT132330.1350894588673372No Hit
GAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGG129530.13223107086137828No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG129010.13170022737455733No Hit
TAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTT128720.13140418004536875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC128570.13125105211647808No Hit
GATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATG128560.13124084358788538No Hit
AACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACT128560.13124084358788538No Hit
GTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT128100.13077125127262068No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACAGGATCCGGAGCTGGT126550.12918892934075057No Hit
AGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTAC125620.1282395361816285No Hit
TTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACA125530.12814765942429412No Hit
TGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTC124910.12751473065154606No Hit
GTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACT124120.12670825689272194No Hit
TGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAG123830.12641220956353333No Hit
TCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACT123710.12628970722042082No Hit
CTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAG123550.12612637076293745No Hit
ACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCC123080.12564656991908005No Hit
TGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTA122960.12552406757596754No Hit
TTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTG122910.12547302493300397No Hit
CTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGC122600.12515656054662996No Hit
GATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGAT122140.12468696823136527No Hit
ACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTA121950.12449300618810379No Hit
CACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTT121180.12370694948646507No Hit
AAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTG121040.12356403008616712No Hit
TAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATAT120580.12309443777090245No Hit
TTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTA120570.12308422924230975No Hit
TTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACC120180.12268609662719403No Hit
GATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAG119980.12248192605533981No Hit
CAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCT118960.12144065613888336No Hit
AAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAA118950.12143044761029065No Hit
CACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTT118080.12054230562272483No Hit
CAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTT117960.12041980327961231No Hit
TGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCA117160.11960312099219547No Hit
CCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCT116510.11893956663366931No Hit
AGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATCAGCAGTCTAGTTC115760.118173926989216No Hit
TTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCT114840.11723474235868665No Hit
ACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTC114770.11716328265853769No Hit
TCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGC114310.11669369034327301No Hit
CAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAAT113780.11615263832785935No Hit
TTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTT113700.11607097009911765No Hit
GCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTA113670.11604034451333954No Hit
TATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCT111450.11377405116575783No Hit
GTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTT111190.11350862942234735No Hit
GCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGT111100.11341675266501294No Hit
ATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGA110960.11327383326471502No Hit
ACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATG110360.11266132154915237No Hit
ATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATT108930.11120150196039479No Hit
ATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGG108650.1109156631597989No Hit
TGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAA108370.11062982435920302No Hit
TGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACAC108040.11029294291564355No Hit
GACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCT107890.11013981498675289No Hit
CTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGT107250.10948646915681942No Hit
AATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAA107130.10936396681370691No Hit
TGCCTTCCTCCTTATCCTACAGTACAATCAGCAGTCTAGTTCTTTTCATT107000.10923125594200168No Hit
TATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGAT106800.10902708537014746No Hit
TAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGG105940.10814915191117436No Hit
GACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTA105860.10806748368243269No Hit
GTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTG105740.10794498133932016No Hit
TTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTA104840.10702621376597622No Hit
TGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCT104370.10654641292211883No Hit
CACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACA104370.10654641292211883No Hit
CTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGA103930.10609723766403956No Hit
GCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTG103500.10565827093455302No Hit
CTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTAT101850.1039738637167558No Hit
CCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCT100410.1025038355994055No Hit
ACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTAC100200.10228945649895858No Hit
ACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTG99900.10198320064117726No Hit
CTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTAC99750.1018300727122866No Hit
TACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTC99690.10176882154073034No Hit
AGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATCA99650.1017279874263595No Hit
GTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACC98850.10091130513894267No Hit
TGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACT98380.10043150429508527No Hit
CTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTG98010.10005378873715498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA950200.0140.771428
GTGGTAT951200.0140.646156
TGGTATC953000.0140.410697
GTATCAA962100.0138.970389
AAGCAGT964400.0138.666551
CAGTGGT969800.0137.977574
AGCAGTG980700.0136.394032
GCAGTGG987750.0135.471543
AGTGGTA1036550.0129.051385
TCAACGC941900.028.6454610-14
ATCAACG942700.028.58296410-14
CAACGCA951650.028.36256810-14
TACATGG949950.028.3314720-24
AGAGTAC950950.028.29259115-19
GTACATG953350.028.2319420-24
CGCAGAG954750.028.19808215-19
CAGAGTA957000.028.16037615-19
GAGTACA949650.028.0871920-24
AGTACAT958350.028.0350620-24
GCAGAGT966700.027.87334315-19
Produced by FastQC (version 0.11.5)