FastQCFastQC Report
Sun 19 Mar 2023
SRR4895853_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895853_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5983110
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG2153850.0143.608473
TGTTCTA6002300.0143.538962
GTTCTAT2102950.0143.133383
GTTCTAA1740950.0143.08563
GTGTTCT6033500.0142.79671
TTCTATG771450.0140.537034
TCTAGTA230000.0140.243075
TTCTAGT784400.0140.190324
TCTAGGT283500.0139.656745
TCTAGTG263600.0139.438145
TCTATGA208900.0139.381125
TTCTAAG636600.0139.136264
TCTAAGG230550.0139.127765
TTCTAGG794050.0139.030644
TCTATGG278400.0138.620295
CTAGTAG75250.0138.258746
CTAGGTA86350.0137.996126
TCTATAG212900.0137.979875
CTAGTGA71850.0137.886876
TCTAAGA166650.0137.821385
Produced by FastQC (version 0.11.5)