FastQCFastQC Report
Sun 19 Mar 2023
SRR4895853_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895853_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5983110
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG284980.47630747220091224No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT198820.3323020970699185No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC188630.3152708206935858No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124660.20835318087081803No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA103420.1728532485613669No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGGTGTGCAACTACCGC91220.1524625153139421No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC89630.14980503450546623No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG77700.129865571584009No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG67480.11278415406034654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA767450.0140.950268
TGGTATC770600.0140.42087
GTGGTAT770500.0140.401666
GTATCAA777500.0139.10989
AAGCAGT778800.0138.935471
CAGTGGT784000.0138.098894
AGCAGTG797200.0135.78632
GCAGTGG798950.0135.497883
AGTGGTA843600.0128.228045
TCAACGC763450.028.60181210-14
ATCAACG763850.028.55855810-14
CAACGCA766800.028.48624410-14
TACATGG767700.028.38157520-24
AGAGTAC770700.028.26549115-19
GTACATG771300.028.23790220-24
CGCAGAG771850.028.21591415-19
AGTACAT773000.028.13295620-24
CAGAGTA775250.028.13117615-19
GAGTACA769900.028.00869620-24
GCAGAGT783900.027.80606715-19
Produced by FastQC (version 0.11.5)