FastQCFastQC Report
Sun 19 Mar 2023
SRR4895852_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895852_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2599467
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGTC2604850.0143.699981
TCTGTCA2605800.0143.64762
CTGTCAG925700.0143.511263
CTGTCAT930900.0143.343843
CTGTCAA745150.0143.334553
TGTCATG338750.0142.683474
TGTCAAG263950.0142.555534
TGTCAGG335250.0142.49794
TGTCAGT327100.0142.438434
TGTCATA262300.0142.29944
TGTCAAT265750.0141.996354
TGTCAGA253250.0141.925834
GTCATGA93550.0141.846255
GTCAGTG110650.0141.788865
GTCATAG92700.0141.671165
GTCAAGT92550.0141.667375
GTCAGGT115650.0141.510975
GTCAGAG89850.0140.79595
GTCATAA79700.0140.568365
GTCATGG120750.0140.542865
Produced by FastQC (version 0.11.5)