FastQCFastQC Report
Sun 19 Mar 2023
SRR4895852_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895852_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2599467
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG139530.5367638827498099No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC90400.3477635992301499No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG70320.2705169944453997No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG62460.24028002663622963No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT53230.20477274764403625No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT52790.20308009295751783No Hit
CCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTG50610.19469375837431288No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA49380.189962019137No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC48800.18773079250477118No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT48570.18684599573681834No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC47860.18411466658357273No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA47790.1838453806107175No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT47650.1833068086650071No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC47050.18099864318339107No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT46970.18069088778584225No Hit
ATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCT46260.1779595586325966No Hit
TTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATAC45900.17657465934362698No Hit
CCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTC45340.17442037156078535No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT45280.17418955501262373No Hit
TGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAAC45040.17326628881997733No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT44950.17292006399773494No Hit
AATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTC44340.17057342909142528No Hit
ACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGT44110.16968863232347248No Hit
AAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATC43930.16899618267898767No Hit
TACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCT43570.16761128339001802No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT42890.16499536251085317No Hit
AGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTT42450.16330270782433476No Hit
ATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCC42400.16311036070086676No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC41990.16153311428842912No Hit
TGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCAT41970.16145617543904192No Hit
AAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATAC41510.15968658190313628No Hit
TGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCC41090.15807086606600507No Hit
CTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAA41080.15803239664131147No Hit
ACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTT40950.15753229412029465No Hit
TATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGAC40870.15722453872274586No Hit
ATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATA40650.15637821137948665No Hit
CTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCT40620.15626280310540583No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT40250.15483943439174264No Hit
GGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACG40020.1539546376237898No Hit
TCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCA39080.15033851170259133No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT38980.14995381745565534No Hit
TACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAG38890.14960759263341294No Hit
CTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTT38500.1481072850703625No Hit
TTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGA38370.1476071825493457No Hit
TTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTAC38280.1472609577271033No Hit
TTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATG38270.1472224883024097No Hit
AAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGA38100.14656850808261848No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37820.14549136419119765No Hit
AGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC37680.14495279224548724No Hit
GGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACC37420.14395258720345364No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG37090.1426830961885648No Hit
AGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGA36980.1422599325169352No Hit
CTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTT36940.1421060548181608No Hit
TGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTG36840.1417213605712248No Hit
TAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTT36730.1412981968995952No Hit
GCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAG36680.1411058497761272No Hit
TGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTT36670.1410673803514336No Hit
TGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTAT36340.13979788933654477No Hit
AAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTA36170.13914390911675356No Hit
CACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTT36100.13887462314389834No Hit
ATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACA36070.13875921486981754No Hit
TCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACT35880.13802829580063913No Hit
AACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACT35610.1369896213339119No Hit
GTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATA35440.1363356411141207No Hit
TAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATAT35250.13560472204494228No Hit
CTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAAT35040.13479686412637668No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG34910.13429676160535986No Hit
GAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTT34650.13329655656332623No Hit
TGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTA34220.13164237130150142No Hit
TTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACA34210.13160390187680782No Hit
TGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAG34100.13118073820517823No Hit
TTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTA34060.13102686050640383No Hit
TGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTC33860.1302574720125318No Hit
TTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTG33690.12960349179274058No Hit
GATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATG33360.12833400077785176No Hit
CACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTT33150.12752614285928615No Hit
ACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCC33090.12729532631112456No Hit
GTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAA33080.12725685688643096No Hit
AGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTAC32820.12625665184439736No Hit
TCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGC32510.1250640996788957No Hit
TTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACC32310.1242947111850237No Hit
CTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAG32190.12383307808870049No Hit
ACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTA32170.12375613923931328No Hit
AAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTG31980.12302522017013488No Hit
TTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTT31940.12287134247136047No Hit
CACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTT31820.12240970937503727No Hit
CAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCT31780.12225583167626286No Hit
CAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTT31410.12083246296259965No Hit
TATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCT31380.12071705468851884No Hit
GAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGG31210.12006307446872763No Hit
GTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT31170.11990919676995322No Hit
CTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGC31130.11975531907117883No Hit
TGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACAC30970.11913980827608121No Hit
AGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATCAGCAGTCTAGTTC30860.11871664460445161No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC30800.11848582805629No Hit
CTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGT30760.11833195035751559No Hit
AAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAA30720.11817807265874118No Hit
GATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAG30700.118101133809354No Hit
TTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCT30490.11729327589078838No Hit
CCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCT30460.11717786761670758No Hit
ACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTC30180.11610072372528676No Hit
ATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGG30110.11583143775243156No Hit
TGCCTTCCTCCTTATCCTACAGTACAATCAGCAGTCTAGTTCTTTTCATT30000.11540827408080195No Hit
TGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCA30000.11540827408080195No Hit
ACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATG29780.11456194673754273No Hit
TAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGG29590.11383102766836432No Hit
GTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACT29550.11367714996958991No Hit
GATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGAT29360.11294623090041152No Hit
ATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGA29300.1127154143522499No Hit
TATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGAT29260.1125615366534755No Hit
CAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAAT28980.11148439276205468No Hit
TGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCT28870.11106122909042507No Hit
AATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAA28790.11075347369287626No Hit
CTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGA28580.10994561577431065No Hit
GTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTT28460.10948398267798744No Hit
TACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTC28420.10933010497921306No Hit
TTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTA28180.10840683878656664No Hit
TGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAA28070.10798367511493702No Hit
ATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATT28060.10794520569024342No Hit
CACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACA27770.10682959237412899No Hit
GCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTA27570.106060203880257No Hit
CCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCT27530.1059063261814826No Hit
GCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGT27450.10559857078393378No Hit
GACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCT27440.1055601013592402No Hit
TAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCA27270.10490612113944899No Hit
CTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTAC27220.10471377401598096No Hit
GTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTG27200.10463683516659376No Hit
GCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTG26930.10359816069986656No Hit
TGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACT26770.10298264990476894No Hit
CTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTAT26550.10213632256150973No Hit
ACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTG26530.10205938371212252No Hit
TCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAA26420.10163622004049293No Hit
ATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAA26400.10155928119110572No Hit
CTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTG26390.10152081176641212No Hit
TGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGC26070.10028979017621688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT264700.0140.954546
GGTATCA265400.0140.79718
TGGTATC266050.0140.426067
GTATCAA267750.0139.615119
AAGCAGT270000.0138.32361
CAGTGGT273000.0136.79044
AGCAGTG275650.0135.475362
GCAGTGG275650.0135.449233
AGTGGTA294450.0126.813345
TCAACGC263100.028.62451410-14
ATCAACG263550.028.54831910-14
CAACGCA264800.028.48968910-14
TACATGG264950.028.43007920-24
AGAGTAC266500.028.26472715-19
CAGAGTA267600.028.20773115-19
GTACATG268900.028.04458620-24
CGCAGAG268850.027.9855315-19
GAGTACA267250.027.93755120-24
AGTACAT270500.027.809520-24
GCAGAGT272150.027.7202615-19
Produced by FastQC (version 0.11.5)