FastQCFastQC Report
Sun 19 Mar 2023
SRR4895851_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895851_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11010422
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGA11016350.0143.921922
GTCGTAG11016600.0143.918641
CGTAGAG4039700.0143.712743
CGTAGAT3884300.0143.682723
CGTAGAA3077600.0143.587943
GTAGATG1468000.0142.234044
GTAGAAG1161500.0141.303184
GTAGATA1123000.0141.268454
GTAGAGG1541900.0140.829074
GTAGAGA1110100.0140.627044
GTAGAGT1371850.0140.614494
GTAGAAT1102900.0139.514794
GTAGAAA853200.0138.176934
TAGATAG418050.0137.66175
TAGATGG561300.0137.62455
TAGATGA405900.0137.596165
TAGAGTA417050.0137.542945
TAGAGTG487650.0137.06035
TAGATTG492250.0137.052025
TAGAGGA438850.0135.94415
Produced by FastQC (version 0.11.5)