FastQCFastQC Report
Sun 19 Mar 2023
SRR4895850_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895850_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4609866
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTC4614350.0143.85892
AATCCGT4621850.0143.625461
TCCGTCG1895000.0143.000093
TCCGTCA1290250.0142.805163
CCGTCTA406300.0141.9264
CGTCGAT188050.0141.816965
CCGTCAG435350.0141.78324
TCCGTCT1452750.0141.764143
CCGTCGA525200.0141.668814
CCGTCAA387750.0141.548284
CCGTCGT684050.0141.54694
CGTCATA127700.0140.841955
CGTCAGT152950.0140.704165
CGTCAAG134200.0140.512025
CGTCGTA198500.0140.44475
CCGTCTG506250.0140.344254
CGTCAAT138150.0140.246925
CGTCGAG174700.0140.166495
CGTCTAG147650.0140.1475
CCGTCAT456700.0139.837314
Produced by FastQC (version 0.11.5)