FastQCFastQC Report
Sun 19 Mar 2023
SRR4895849_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895849_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8488531
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACAC8513600.0143.575152
AAGGACA8516100.0143.532991
GGACACT2788350.0143.460023
GACACGT1153250.0143.144364
GACACTG965100.0143.07464
GACACTA834750.0142.714514
GGACACG3278400.0142.620473
GACACAT857300.0142.479574
GACACGA913200.0142.28094
GACACTT944400.0142.147084
GGACACA2463750.0142.09433
GACACGG1131000.0141.625154
ACACGGT399350.0140.970785
GACACAG839500.0140.70634
ACACGTA355550.0140.334385
ACACGTG377200.0140.029395
ACACTGG341450.0139.761295
ACACGAT328600.0139.486015
ACACGTT415050.0138.344475
ACACTGA268200.0138.227875
Produced by FastQC (version 0.11.5)