FastQCFastQC Report
Sun 19 Mar 2023
SRR4895847_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895847_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10541562
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAC10601250.0143.188671
ACTCACC10616350.0142.9852
CTCACCG4069800.0142.739973
TCACCGA1125550.0142.669054
TCACCGT1428600.0142.109634
TCACCTG1212000.0141.255054
TCACCGG1426100.0141.111724
TCACCAG1049250.0140.760714
CTCACCA3065750.0140.584843
CTCACCT3555000.0140.556563
TCACCTA1028200.0139.959154
CACCGAT401800.0139.627275
ACCGGTA153450.0138.744486
CACCGTG487100.0138.69315
TCACCAT1077250.0138.686084
CACCGGT496750.0138.680225
ACCTAGT125000.0138.412416
CACCGTA425700.0138.164525
ACCGATG139000.0138.042776
CACCGAG386650.0137.724175
Produced by FastQC (version 0.11.5)