FastQCFastQC Report
Sun 19 Mar 2023
SRR4895846_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895846_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338748
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA3343450.0143.795212
CACGCAT1177700.0143.608523
TTCACGC3349350.0143.541921
ACGCATG419750.0143.48524
CACGCAG1194350.0143.252273
ACGCATA336900.0143.144934
ACGCATT397700.0142.841134
CACGCAA971250.0142.665443
ACGCAGT408750.0142.608234
ACGCAGG434750.0142.476174
ACGCAAT334650.0142.429184
ACGCAAG336550.0142.416674
ACGCAGA325850.0142.121634
CGCATAG120250.0141.84435
CGCAATG121550.0141.45275
CGCAGTA124150.0141.216065
CGCAGTT138050.0141.131275
CGCATGG154050.0141.05535
CGCATTG148800.0141.048195
CGCAAGT108700.0140.886645
Produced by FastQC (version 0.11.5)