FastQCFastQC Report
Sun 19 Mar 2023
SRR4895846_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895846_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338748
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG468621.4035800246080266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC338381.0134936808648032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36800.1102209570773236No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGAGGACCTGATGACCAG36580.10956202744262221No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAGAGGACCTGATGACCA35900.10752533584445427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA520350.0140.512548
TGGTATC521200.0140.324837
GTGGTAT523950.0139.574576
GTATCAA524650.0139.347189
AAGCAGT527850.0138.577731
CAGTGGT533700.0137.064044
AGCAGTG537150.0136.187792
GCAGTGG542000.0134.99573
AGTGGTA626250.0116.7516255
TCAACGC517050.028.62442810-14
CAACGCA517850.028.58854910-14
ATCAACG516700.028.58529310-14
AGAGTAC519750.028.35659415-19
TACATGG520200.028.337620-24
CGCAGAG522000.028.25919515-19
CAGAGTA523900.028.18144615-19
GTACATG524200.028.14059620-24
AGTACAT524750.028.04798520-24
GAGTACA522300.027.92039120-24
GCAGAGT533300.027.6982215-19
Produced by FastQC (version 0.11.5)