FastQCFastQC Report
Sun 19 Mar 2023
SRR4895845_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895845_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096118
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG4098200.0143.925261
TCGTAGA4098750.0143.905942
CGTAGAG1510200.0143.686143
CGTAGAT1437850.0143.650283
CGTAGAA1143650.0143.597893
GTAGATG540700.0142.25644
GTAGATA413200.0141.70074
GTAGAAG437650.0141.35214
GTAGAGT504500.0140.589894
GTAGAGG586250.0140.512864
GTAGAGA409600.0140.256654
TAGATGA150400.0138.734835
GTAGAAT406700.0138.618934
TAGATAG150850.0138.559625
TAGATGG207150.0137.674935
GTAGAAA316150.0137.100254
TAGAGTG185100.0136.999155
TAGAGTA149700.0136.642045
TAGATTG177550.0136.620325
GTAGATT487900.0135.958134
Produced by FastQC (version 0.11.5)