FastQCFastQC Report
Sun 19 Mar 2023
SRR4895845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096118
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG156540.3821667246890837No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG109930.2683760575256865No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG98820.24125281547064803No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC97520.23807907877653914No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC88330.21564320168510773No Hit
CCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTG87820.2143981203666496No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC87110.21266477186448243No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC84840.20712293932938453No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT84750.20690321909671547No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA84020.20512104387617738No Hit
AAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATC81400.19872474376958865No Hit
ATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCT81390.19870033041040322No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC80520.19657636816126878No Hit
CCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTC79420.19389089865086895No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT79250.19347587154471624No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT78500.19164486960580726No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT77810.189960347822011No Hit
TTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATAC76680.18720163823405478No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA76220.18607862371152395No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT75490.18429644849098584No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT73950.18053679117642607No Hit
TACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCT73320.17899874954774253No Hit
ATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATA71890.1755076391842227No Hit
AATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTC71400.17431138458413553No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT70850.1729686498289356No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC69710.17018552688179395No Hit
ACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTT69430.16950195282460123No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT69040.16854983181636857No Hit
ACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGT68690.16769536424487771No Hit
AAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATAC68220.1665479363631614No Hit
ATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCC68150.16637704284886323No Hit
TTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTAC68020.16605966917945233No Hit
TGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAAC67850.16564464207329965No Hit
TCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCA67320.16435073403647063No Hit
GGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACG67220.16410660044461609No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT67040.16366715997927794No Hit
CTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCT66460.16225118514652165No Hit
TGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCAT66450.16222677178733622No Hit
CTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAA65730.1604690099259836No Hit
TGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCC64880.15839387439522007No Hit
TTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGA64840.15829622095847826No Hit
TATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGAC64020.1562943255052711No Hit
CACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTT63680.1554642712929657No Hit
AGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTT63420.1548295239541439No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT62300.15209522772537315No Hit
GTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATA62040.15146048038655135No Hit
ATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACA61920.15116752007632592No Hit
CTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTT61880.1510698666395841No Hit
AAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGA61630.15045953265994777No Hit
AGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGA61030.14899473110882058No Hit
CTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTT60640.14804261010058792No Hit
TTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATG60430.1475299295576934No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG59860.1461383680841226No Hit
AGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC59720.14579658105552623No Hit
TTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACA59600.1455036207453008No Hit
AAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTA58950.14391675239824633No Hit
TGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTAT58840.14364820544720636No Hit
TAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATAT57990.14157306991644283No Hit
TGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTT57920.14140217640214467No Hit
TGCCTTCCTCCTTATCCTACAGTACAATCAGCAGTCTAGTTCTTTTCATT57880.14130452296540286No Hit
TCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACT57680.1408162557816938No Hit
AACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACT57630.14069418898576652No Hit
GGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACC57600.14062094890821017No Hit
ACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTA57510.14040122867554108No Hit
TACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAG57390.14010826836531565No Hit
AGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATCAGCAGTCTAGTTC57350.14001061492857383No Hit
TGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTG56940.13900966720197028No Hit
ACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCC56850.1387899469693012No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG56480.13788665267943942No Hit
GTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAA56060.13686129159365037No Hit
TTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTA54970.13420023544243598No Hit
CTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAAT54620.13334576787094513No Hit
CTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAG54490.13302839420153423No Hit
GCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAG54390.13278426060967968No Hit
TGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTC54350.1326866071729379No Hit
TGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAG54220.132369233503527No Hit
TAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTT54200.13232040678515608No Hit
TCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGC54170.13224716670759973No Hit
CTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGC54130.13214951327085792No Hit
TTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTG53400.13036733805031983No Hit
CAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAAT53310.13014761781765077No Hit
AGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTAC52700.12865840290733813No Hit
CACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTT52450.1280480689277018No Hit
GTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT52320.1277306952582909No Hit
CAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTT52220.12748656166643638No Hit
CCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCT52210.12746214830725092No Hit
TGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCT52190.12741332158888002No Hit
GAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTT51720.1262658937071637No Hit
TGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTA51590.1259485200377528No Hit
CACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACA51530.1258020398826401No Hit
GTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACT51500.12572879980508375No Hit
AAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTG51480.12567997308671283No Hit
TGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCA51280.12519170590300377No Hit
CAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCT51080.1247034387192947No Hit
TTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACC50830.12409310473965839No Hit
GATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATG50700.1237757310702475No Hit
TTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTT50630.12360483755594932No Hit
CACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTT50320.12284802342120027No Hit
AAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAA50300.12279919670282938No Hit
GAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGG49980.12201796920889488No Hit
GATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGAT49160.1200160737556877No Hit
ACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTC48970.11955221993116408No Hit
TACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTC48950.11950339321279317No Hit
ATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGG48820.11918601954338229No Hit
TATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCT48560.1185512722045605No Hit
CTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTAT48300.11791652486573873No Hit
AGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATCA47980.11713529737180423No Hit
CTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTAC47660.11635406987786973No Hit
TGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACAC47640.11630524315949882No Hit
GCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTA47580.1161587630043861No Hit
CCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCT47340.11557284238393523No Hit
ACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATG47300.11547518894719341No Hit
TTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCT47170.11515781527778252No Hit
CTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGT46950.11462072137570255No Hit
GATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAG46770.11418128091036439No Hit
ACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTAC46300.1130338530286481No Hit
GTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTT46290.11300943966946264No Hit
ATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGA46260.11293619959190629No Hit
TGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAA46060.11244793240819723No Hit
GACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTA45790.11178877171018999No Hit
CTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGA45720.11161787819589182No Hit
ATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATT45600.11132491788566638No Hit
TATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGAT45160.11025073008150645No Hit
TGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATCAGCAGTCTAG44440.10849296822015382No Hit
TAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGG44050.10754084721192114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43610.1064666594077612No Hit
AATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAA43540.10629576589346304No Hit
GCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGT43370.10588073878731033No Hit
GTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTG43260.10561219183627035No Hit
CTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTG43120.10527040480767401No Hit
CTTCCTCCTTATCCTACAGTACAATCAGCAGTCTAGTTCTTTTCATTTGG42940.10483096434233583No Hit
GACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCT42880.1046844841872231No Hit
ACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTG42450.10363470974224863No Hit
ATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAA42140.10287789560749958No Hit
TGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACT42110.10280465552994322No Hit
GCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTG42100.10278024217075776No Hit
TTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTA41680.10175488108496873No Hit
CGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGT41560.1014619207747433No Hit
TCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAA41230.10065627992162335No Hit
GTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACC41010.10011918601954338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA401000.0140.587148
GTGGTAT403100.0139.83866
TGGTATC403850.0139.630687
GTATCAA406450.0138.64899
AAGCAGT407800.0138.108471
CAGTGGT412050.0136.946914
AGCAGTG415750.0135.572252
GCAGTGG417600.0135.075133
AGTGGTA441850.0127.674165
TCAACGC398650.028.56492410-14
ATCAACG398750.028.51442710-14
CAACGCA399850.028.482810-14
AGAGTAC400950.028.32205615-19
TACATGG400400.028.321420-24
GTACATG401550.028.24746320-24
CAGAGTA403750.028.14347515-19
AGTACAT403650.028.0648320-24
CGCAGAG405050.028.02115415-19
GAGTACA403000.027.8885620-24
GCAGAGT410200.027.69392415-19
Produced by FastQC (version 0.11.5)