FastQCFastQC Report
Sun 19 Mar 2023
SRR4895844_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895844_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4955239
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTA4983350.0143.185932
GTGGTAG1894000.0142.653633
GTGGTAA1410100.0142.508393
TGGTGGT5025550.0141.983581
GTGGTAT1695550.0141.808213
TGGTATA447950.0140.463264
TGGTAGG694550.0139.894264
TGGTAAG514100.0139.853874
TGGTAGA473950.0139.760944
TGGTATG628050.0139.654484
TGGTAGT609900.0138.89954
TGGTAAT482900.0138.885274
TGGTAGC160250.0137.70924
GGTATGC94100.0137.342625
GGTAGTC86600.0136.1015
GGTATAG167850.0135.506095
GGTATAC50200.0135.250385
GGTAATG175750.0135.150455
TGGTAAA384150.0135.00294
GGTAGGC151500.0134.732075
Produced by FastQC (version 0.11.5)