FastQCFastQC Report
Sun 19 Mar 2023
SRR4895844_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895844_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4955239
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT398550.804300256758554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG342970.6921361411629187No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA232480.46916001427983595No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC230540.46524496598448634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC189850.38312985508872527No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC86760.1750874175796566No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT71080.1434441406357998No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG58660.1183797592810357No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG55850.11270899345117359No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGGTGTGCAACTACCGC55170.11133670848166961No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC51720.10437438032756846No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49930.10076204195196235No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT49830.1005602353388No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT49710.10031806740300518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA931700.0141.43318
TGGTATC933750.0141.184287
GTGGTAT934600.0141.035616
AAGCAGT938100.0140.500231
GTATCAA939100.0140.287969
CAGTGGT950800.0138.699624
GCAGTGG963400.0136.894453
AGCAGTG964150.0136.789352
AGTGGTA1010650.0130.415765
TCAACGC928050.028.6725110-14
ATCAACG928800.028.6152510-14
CAACGCA930300.028.60161610-14
TACATGG932550.028.45848820-24
AGAGTAC934950.028.38851715-19
CGCAGAG935750.028.35809115-19
CAGAGTA939950.028.27121215-19
GTACATG939150.028.25542620-24
GCAGAGT944650.028.13207215-19
AGTACAT942350.028.10141220-24
GAGTACA934800.028.08498820-24
Produced by FastQC (version 0.11.5)