FastQCFastQC Report
Sun 19 Mar 2023
SRR4895843_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895843_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221134
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCA2227250.0143.601352
GCTTCAG837550.0143.417653
GCTTCAT761750.0143.123173
TGGCTTC2239100.0142.841371
GCTTCAA627800.0142.488333
CTTCAGT291200.0141.999444
CTTCATG285750.0141.835244
CTTCAGA224000.0141.720034
CTTCAGG314050.0141.136374
CTTCATA223450.0139.781074
CTTCAAT222200.0139.044434
TTCAGGA85200.0139.016225
CTTCAAG235400.0138.924824
TTCAGTA84600.0138.725525
TTCATGA72900.0138.66885
TTCAGAG81850.0138.63625
TCAGTGA27750.0138.034556
TTCATGG113900.0137.996845
TTCATAG84000.0137.059255
TCAAGTA22600.0136.993266
Produced by FastQC (version 0.11.5)