FastQCFastQC Report
Sun 19 Mar 2023
SRR4895843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221134
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT480622.163849637167321No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA252601.1372569147111342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC159370.7175163677652947No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125730.566062200659663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT107700.4848874493839634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA84210.3791306602843412No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA80750.3635530319197311No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAATCTTAGTTCAACTTTA64310.2895367861641846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC62540.2815678837926933No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT50020.22520028057739874No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGGGGGTCCCAAGGA44240.19917753723998644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGCCCGCGCGGCGTGAA41920.18873242226718426No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT38540.1735149702809466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG37520.16892272145669734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCTCAGCTGTCAATGTG37200.16748201594320739No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGGGGCGGGCGCCGG37090.1669867734229452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAACCCCGTGGTCTCCTACG36300.16343003168651687No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTCTCCTACGCCGTGGCTC36090.1624845686932891No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACCCGTCTATGTA35390.15933302538252983No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAGCCTGGTGATAGCTGGT32650.14699698442327208No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGCAAAAAAAAAAAAAAAA32610.14681689623408584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGCCGCAGCACCACTGAC32380.14578138914626493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACTCAAAAAAAAAAAAAAA31660.14253980174091252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGGGGGGATGCGTGCATT31390.14132420646390537No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAACAGCTAAGGACTGCAAA28760.12948340802490982No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCACACCCGTCTATGTAG28240.1271422615654886No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC27880.1255214678628124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCGCGATGTGCGCTTCGAG27040.12173961588990129No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCACTCTGCCGCCGCAGCAC26200.11795776391699014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAACAGCTAAAAGAGCACAC25650.11548155131567929No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCCCTGTAATTGGAATG25100.11300533871436844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAACCGGATTGAGAACAGG24950.11233000800492002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGA24830.11178974343736127No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACAAACCTACCGAGCCT24010.10809793555904326No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTAAATCCCCTTGTAAAT23560.106071943430698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCTTCCAACCCGAGGCA23430.10548665681584272No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTCCAAAGAGGAACAGCTC23190.10440612768072524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA22390.10080436389700036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCAATAGATATAGTACCG22260.10021907728214507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1298500.0142.947881
TGGTATC1310000.0141.706487
GTGGTAT1310400.0141.635746
GGTATCA1311100.0141.609548
GTATCAA1313400.0141.345129
AGTGGTA1313400.0141.298975
CAGTGGT1315700.0141.167244
AGCAGTG1318050.0140.885942
GCAGTGG1324300.0140.228743
GCGGTAT1250.069.119216
CGGTATC1250.069.119217
AGCGGTA1650.052.3642165
AGTGGTC4200.034.2860955
GACTGGA9700.030.4381243
TGACTGG10600.029.2131332
GGTATCC4200.029.1425258
TGGAGTT10250.028.7996716
CAACGCA1308050.028.68408210-14
TCAACGC1308650.028.65882510-14
ATCAACG1307350.028.6454710-14
Produced by FastQC (version 0.11.5)