FastQCFastQC Report
Sun 19 Mar 2023
SRR4895842_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895842_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456836
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT21130.4625292227407647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTGC457350.0144.029632
CGGATTG457900.0143.856631
GATTGCT155900.0143.78683
ATTGCGA42050.0143.688644
GATTGCG158300.0143.656463
GATTGCA132350.0143.603793
ATTGCGT58100.0142.962054
ATTGCAG48200.0142.258394
ATTGCAT49550.0142.165864
ATTGCTA43600.0141.557114
ATTGCGG56700.0141.405464
ATTGCTG58950.0141.0234
GATTGCC12150.0140.642473
TTGCAGT15350.0140.445315
TTGCTGA16700.0140.317325
TTGCGAA12700.0139.661225
TTGCGAT15100.0139.42815
TTGCGTA15000.0139.396275
ATTGCTT54600.0139.317054
ATTGCAA35400.0139.31484
Produced by FastQC (version 0.11.5)