FastQCFastQC Report
Sun 19 Mar 2023
SRR4895841_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895841_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602574
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACA604050.0143.855731
GACACAC605200.0143.582372
CACACTG69450.0141.929674
ACACACG178250.0141.705783
ACACACT214200.0141.621753
ACACTAG21200.0140.132315
CACACGA58450.0139.772694
CACACGT54650.0139.727774
CACACGG65800.0139.063354
ACACGAT19550.0139.050175
CACACTT80050.0138.80924
CACACTA65050.0138.671424
ACACTGT26000.0138.389825
ACACTGA18950.0138.125665
ACACGGT21350.0137.796835
CACACAT68950.0137.729974
CACACAG66200.0137.569554
ACACGTG17000.0137.42735
ACACGTA15650.0137.302635
ACACTGG23200.0136.44375
Produced by FastQC (version 0.11.5)