FastQCFastQC Report
Sun 19 Mar 2023
SRR4895840_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895840_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916420
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATC919650.0143.698792
CTATCGG111800.0143.627994
CCTCTAT920250.0143.60511
TCTATCG321100.0143.579683
CTATCAG92850.0143.431854
CTATCGA90750.0143.254974
CTATCGT115000.0143.079834
CTATCTG111700.0142.594644
TCTATCA263500.0142.429753
TCTATCT313100.0141.97463
CTATCTA91350.0141.524734
CTATCAT94000.0141.063464
TATCGAG30650.0140.914925
CTATCAA76100.0140.70274
TATCGGA26600.0140.684545
TATCGGT39050.0140.330875
TATCGAT31250.0140.055225
TATCAGT32550.0139.999575
TATCGTG40400.0139.925025
TATCAGA25050.0139.890815
Produced by FastQC (version 0.11.5)