FastQCFastQC Report
Sun 19 Mar 2023
SRR4895840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916420
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92181.005870670653194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG68780.7505292333209664No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG19000.20732851749197967No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC12820.13989218917090418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA11760.128325440300299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT172300.0111.655431
GTGGTAT172450.0111.251836
GGTATCA173300.0111.166398
TGGTATC174500.0110.111067
AGTGGTA174950.0109.715485
GTATCAA176000.0109.4018869
AGCAGTG176950.0108.727232
CAGTGGT177950.0108.034714
GCAGTGG182150.0105.304793
TATCGGG651.092232E-555.7807775
TCTATCG1750.053.871233
CTCTATC4650.048.3459782
CCTCTAT5300.045.1508561
CTATCGT855.332906E-542.6582154
CTATCGG1453.4838537E-530.0078474
CGTACTT1504.3986496E-528.999672145
TGCGGCT6650.026.16512145
CGATAGG2350.024.68272835-39
CATGGGG66850.024.2929225-29
CAACGCA172950.023.5069810-14
Produced by FastQC (version 0.11.5)