FastQCFastQC Report
Sun 19 Mar 2023
SRR4895839_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895839_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778248
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAATCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG117551.5104439715874631No Hit
CCTAATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC22430.28821146986564694No Hit
CCTAATCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT14950.19209814866212313No Hit
CCTAATCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCG14040.18040521787399388No Hit
CCTAATCCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGC10210.1311921135679115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATC780200.0143.858951
CTAATCC780400.0143.822082
TAATCCG257000.0143.667473
AATCCGT92850.0142.9864
TAATCCA246600.0142.708013
AATCCGA70850.0142.70194
AATCCGG92200.0142.586154
ATCCGTA27950.0142.164575
AATCCAG77950.0141.73084
ATCCATG56200.0141.662345
TAATCCT256200.0141.132433
AATCCTG90200.0141.03074
ATCCGGA24700.0141.01715
ATCCGAT24700.0141.01715
AATCCAT105100.0140.935154
ATCCTAG27800.0140.856435
ATCCGGT29750.0140.835055
ATCCGAG23450.0140.538385
ATCCGTG31450.0140.33065
AATCCTA78500.0140.094734
Produced by FastQC (version 0.11.5)