FastQCFastQC Report
Sun 19 Mar 2023
SRR4895838_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895838_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413724
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG151.2558007E-4144.231237
CGATGAG400.0144.231238
CGACAAC414600.0143.900762
CCGACAA414700.0143.866061
GACAACT143250.0143.50913
GACAACG139800.0143.49173
GACAACA121700.0142.140083
ACAACGT48250.0141.82274
CAACTGT17900.0141.813955
ACAACTG51700.0141.563844
CAACGAT14400.0140.725625
ACAACGA41350.0140.028644
ACAACGG51600.0139.602314
CAACGGT16650.0139.466845
CAACGTG15950.0139.257755
AACGGAT5300.0138.788546
CAACTGA14550.0138.77925
CAACTTG18050.0138.637795
CAACAGT15450.0138.163255
CAACTAG14000.0138.04995
Produced by FastQC (version 0.11.5)