FastQCFastQC Report
Sun 19 Mar 2023
SRR4895838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413724
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG48901.1819473852133306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG39880.963927642582978No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38190.9230791542187545No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT14910.3603851843257824No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC10870.26273554350243156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC10420.251858727074088No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA9190.22212876216994906No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT6510.1573512776633698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT6390.15445079328247818No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC5840.14115690653672497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG5450.13173033229882725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC4900.11843644555307403No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC4820.1165027892991463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT117500.0113.583331
GTGGTAT118600.0112.1698156
GGTATCA120000.0111.2840658
TGGTATC119950.0110.967827
GTATCAA120550.0110.896639
CAGTGGT120100.0110.809034
AGCAGTG120850.0110.361392
GCAGTGG121500.0109.472543
AGTGGTA121800.0109.3815165
CCGACAA1350.075.219231
CGGTCTG954.5474735E-1061.050747145
CGACAAC1950.052.0685582
AAATATA11950.047.92739145
GACAACG803.7235284E-545.3275383
CCGCAAG5150.039.41626145
CACGCAG2700.037.59143145
TGCGGCT1801.2896271E-732.22123145
CCGGACG1652.3825396E-630.756626145
TGTAAAC2156.1116407E-726.975914145
TAACCCG556.7757355E-926.362823115-119
Produced by FastQC (version 0.11.5)