FastQCFastQC Report
Sun 19 Mar 2023
SRR4895837_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895837_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686221
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC54860.799450905757766No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG34680.5053765477885404No Hit
CCATCCTCTGTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC9910.1444141173178903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCG239000.0143.510883
CATCCTC690650.0143.24552
ATCCTCA197750.0143.190193
CCATCCT690950.0143.18331
TCCTCGA68450.0141.752234
CCTCATG33200.0141.351765
ATCCTCT234300.0141.159273
TCCTCAG67950.0138.763924
CTCGTAG7700.0138.557256
CTCATGA6150.0138.313676
TCCTCAT77650.0138.14014
CTCGAAT6700.0137.718876
CCTCGAG22300.0137.709215
CCTCGGA22200.0137.355385
CCTCGAT25100.0137.281635
CCTCGTA24000.0137.266085
TCCTCGG90750.0137.184164
CTCGATG8100.0137.054726
TCCTCAA57600.0137.04084
CTCGAGA6450.0136.351036
Produced by FastQC (version 0.11.5)