FastQCFastQC Report
Sun 19 Mar 2023
SRR4895837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686221
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG307874.486455529632582No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61470.8957755591857434No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA28990.42245865399047833No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG17690.25778867157956403No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT8900.1296958268546139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT271350.075.5587841
AGTGGTA278150.073.597345
GGTATCA280900.073.155478
GTGGTAT280700.072.8976066
CAGTGGT281100.072.850784
TGGTATC281950.072.600147
GTATCAA284100.072.4432459
AGCAGTG283350.072.323472
GCAGTGG292250.070.046543
CAGGGGG7850.042.480724145
TGCGGCT4500.032.21968145
CGACCTA1950.023.7933445-49
TACGCAC2250.023.1981750-54
AAATATA15000.022.714874145
ACGTACG403.1037634E-421.751455130-134
CGACGTA604.565063E-721.74828385-89
GCCCCAA7050.021.594042145
CATGGGG48150.021.29280325-29
ATGGGAG26150.020.35185825-29
CGTGGGA3951.411172E-820.188282145
Produced by FastQC (version 0.11.5)