FastQCFastQC Report
Sun 19 Mar 2023
SRR4895836_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895836_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123639
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTTACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3920.3170520628604243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATGG100.007200049144.209968
CGAAATG202.1816722E-6144.209968
CGTTAGC100.007200049144.209968
TACGCAC100.007200049144.209966
CTAGTTA100.007200049144.209968
TACTGAC202.1816722E-6144.209966
GTCGTCT100.007200049144.209969
CGAGATC100.007200049144.209968
CGAGAGT100.007200049144.209968
CGATGGT202.1816722E-6144.209968
ACCGTCT100.007200049144.209967
CTGATGA202.1816722E-6144.209968
TACGTGC100.007200049144.209966
ACTGGTA400.0144.209967
ACAGTAT351.2732926E-11144.209957
CATGATA151.254425E-4144.209958
TACGGAG1150.0144.209956
CGTAGAG151.254425E-4144.209958
CGGGATA253.8144208E-8144.209958
ACGTCGT151.254425E-4144.209957
Produced by FastQC (version 0.11.5)