FastQCFastQC Report
Sun 19 Mar 2023
SRR4895836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123639
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG41143.3274290474688404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11860.959244251409345No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA7590.6138839686506685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC3490.28227339269971446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2910.2353626282968966No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC2180.17631976965197066No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG1790.14477632462248968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGTTAAGAAAAGAAATGAT1540.12455616755230954No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1520.12293855498669513No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1510.12212974870388793No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC1400.11323287959300868No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAATAGAAACTTCAGGC1380.11161526702739427No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGTATCTGAAGAATA1270.102718397916515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCTC100.007085882144.9839
AAGCAGT57550.098.250871
AGTGGTA58150.097.7357565
CAGTGGT59150.095.960854
GTGGTAT59250.095.676556
GGTATCA59450.095.4766168
AGCAGTG59300.095.473632
GCAGTGG59250.095.4318543
GTATCAA59650.095.156499
TGGTATC59800.094.796587
CGGTCTG554.129106E-1092.26191145
CAATGTC353.427338E-582.847435145
CGTGTAC300.001932615472.4915145
CCTATAA300.001932615472.4915145
TCTGGGT554.0434097E-665.90137145
GATATCA606.77519E-660.4095847
CGGGTCC450.00962376948.327667145
AGTGATA855.3179385E-542.6420634
AGCCCCA1458.624593E-734.9959145
GTGATAT1102.425547E-432.9506845
Produced by FastQC (version 0.11.5)