FastQCFastQC Report
Sun 19 Mar 2023
SRR4895835_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895835_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648459
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGAGC650750.0143.66972
AGGAGCT224500.0143.633383
CAAGGAG651750.0143.449251
AGGAGCA187700.0143.25753
GGAGCAT67450.0143.217364
AGGAGCG222250.0143.206193
GAGCGAT21850.0143.189485
GGAGCTA65850.0142.975044
GGAGCGT84550.0142.218234
GAGCTAG21550.0142.172135
GAGCAGT20850.0142.104755
GGAGCTG72700.0141.997744
GAGCGTA24050.0141.781285
GAGCATG22300.0141.59315
GAGCTAA20800.0141.060015
GGAGCGA65950.0141.009314
GAGCGGT24800.0140.981755
GAGCTAT22400.0140.960985
GAGCATA19900.0140.918935
GGAGCTT85800.0140.902484
Produced by FastQC (version 0.11.5)