FastQCFastQC Report
Sun 19 Mar 2023
SRR4895834_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895834_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987516
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGGCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG25340.25660343731139545No Hit
ATTGGCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC14840.15027604616026474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCT990700.0143.74121
TGGCTCA279400.0143.48443
TTGGCTC993250.0143.372182
TGGCTCG339350.0143.335833
GGCTCGA96750.0142.940064
GGCTCAT104450.0142.757324
GCTCATG41200.0142.456915
GCTCGAT32050.0142.407215
GGCTCGT122150.0142.318074
GCTCGTA34300.0142.315065
TGGCTCT340300.0141.961033
GCTCGAA27200.0141.82125
GGCTCAG97050.0141.532354
GCTCGGA29300.0141.500035
GGCTCAA78600.0141.271474
GGCTCTA97000.0141.159324
GCTCTGA32600.0141.110525
GGCTCTG123450.0140.819384
GCTCAGT31800.0140.805885
GCTCATA28000.0140.60185
Produced by FastQC (version 0.11.5)