FastQCFastQC Report
Sun 19 Mar 2023
SRR4895833_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895833_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829136
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG522466.301258177186855No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA103191.2445485420968334No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92671.117669477624901No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT24050.290060979139731No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC16940.20430906389301637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG13660.16474981185233784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT12360.14907084000694698No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA10710.12917060651087398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT458600.071.230791
AGTGGTA469850.069.385055
AGCAGTG473850.068.822942
TGGTATC476600.068.402367
GTGGTAT476450.068.393466
GGTATCA478750.068.292038
GTATCAA479900.068.20399
CAGTGGT479350.068.018154
GCAGTGG481650.067.618083
CGGTCTG3150.050.633804145
TGCGGCT6500.037.92224145
CCGCAAG8450.036.892677145
CATTCCG2451.9699655E-929.592973
CGTGGGG3057.1122486E-1026.146963145
CACGGGA2808.194547E-925.892286145
CGGAGCC3408.913048E-1125.587671145
TACGCAC2550.025.58612850-54
ATCATTC6950.025.039951
CATGGGG61950.024.48240325-29
CGACCTA2550.023.88067445-49
Produced by FastQC (version 0.11.5)