FastQCFastQC Report
Sun 19 Mar 2023
SRR4895832_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895832_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794728
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69140.8699831892169395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG58410.7349684420329974No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC11960.15049174057035866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA10630.13375645503870506No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC9660.12155102122990506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT148000.0108.811131
AGTGGTA148000.0108.356565
GGTATCA148900.0108.334588
GTGGTAT148450.0107.979256
GTATCAA150750.0107.00519
TGGTATC150800.0106.5850147
AGCAGTG152200.0105.754152
CAGTGGT153200.0104.732574
GCAGTGG154100.0104.0738453
TAGCGAC2050.095.492452
ATAGCGA2450.079.906871
CGACGGT350.003566436562.1419645
AGCGACG1301.8189894E-1255.771943
GCGACGG1105.2611795E-639.547384
GGTCTGA5650.034.645523145
AGCGACA1956.9849193E-933.463163
GCGAACC552.1282176E-1028.99958650-54
CGGTCTG3450.027.318449145
GAGGTAT2954.820322E-1027.033516
CGACCTA3350.025.5369545-49
Produced by FastQC (version 0.11.5)