FastQCFastQC Report
Sun 19 Mar 2023
SRR4895831_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895831_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073280
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAC1074500.0144.045752
AGGCTAA1075000.0143.978731
GCTAACG360400.0143.953413
GCTAACT382000.0143.930363
CTAACGA102050.0143.648244
GCTAACA305650.0143.505773
CTAACGT128700.0143.429144
CTAACGG127200.0143.249824
CTAACTG132800.0142.964684
CTAACTA114150.0142.82384
TAACGTG43400.0141.389055
TAACGGT42200.0141.308735
CTAACTT135200.0141.173514
TAACGAT35100.0140.926595
CTAACAT111000.0140.705614
TAACGAG35500.0140.151155
TAACGTA38300.0140.07165
TAACTAG37950.0140.03345
CTAACAG109250.0139.98944
TAACGGA34150.0138.934835
Produced by FastQC (version 0.11.5)