FastQCFastQC Report
Sun 19 Mar 2023
SRR4895831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073280
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104480.9734645199761478No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG74240.6917113893858079No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT23330.2173710494931425No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG22180.2066562313655337No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC16090.14991428145497912No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA14490.1350067084078712No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACGCGCTTCAACTTCGGT12530.11674493142516398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT12410.1156268634466309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT228100.0117.676611
GTGGTAT228850.0117.1216356
GGTATCA230600.0116.610088
AGTGGTA231000.0115.9687655
GTATCAA232350.0115.8200159
TGGTATC231950.0115.618817
AGCAGTG234650.0114.139282
CAGTGGT235900.0113.565214
GCAGTGG239350.0111.746533
CGGTCTG3800.041.971783145
AGCGCAT2200.039.543663145
GCTAACG1859.640644E-1139.1910633
TGCGGCT6250.034.798424145
GGCTAAC7450.028.2228282
CTAACGT1050.007706897327.620374
CGGAGCC6400.027.18627145
CATGGGG83300.025.03007925-29
TACATGG225250.024.55715620-24
CGACCTA4200.024.51193645-49
CAACGCA229650.024.49072810-14
Produced by FastQC (version 0.11.5)