FastQCFastQC Report
Sun 19 Mar 2023
SRR4895830_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895830_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368705
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATGC1370000.0144.078322
AGCCATG1370350.0144.041521
CCATGCG490650.0143.67143
CCATGCT436650.0143.505073
CCATGCA404150.0143.50153
CATGCGA137100.0143.426254
CATGCGT173850.0143.344154
CATGCTG157650.0143.025964
CATGCAG145500.0142.926654
CATGCAT140450.0142.880314
CATGCGG176050.0142.740664
CATGCTA124500.0142.65144
CATGCTT153200.0142.097144
ATGCGAT48950.0142.005545
CATGCAA117450.0141.636614
ATGCAGT46900.0141.293985
ATGCGAG46550.0141.2725
ATGCTGA42950.0140.689545
ATGCGTA52100.0140.616715
ATGCGGT57950.0140.606695
Produced by FastQC (version 0.11.5)