FastQCFastQC Report
Sun 19 Mar 2023
SRR4895830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368705
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109460.7997340551835495No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG84290.6158375983137345No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC29390.21472852075502025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT15070.11010407648105326No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT14960.10930039709068061No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG14840.1084236559375468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA249350.0116.573418
AAGCAGT251500.0116.0254061
GTGGTAT249750.0115.772826
AGTGGTA251650.0115.042765
TGGTATC251700.0115.024117
GTATCAA253500.0114.6566249
AGCAGTG257500.0113.044492
CAGTGGT257500.0112.5699544
GCAGTGG261550.0110.9654543
CGGTCTG4700.040.1052145
GCTATTC10950.031.779886145
GGCTATC7300.030.786762145
GCTATCC6000.030.207443145
CCATGCG4350.028.3345683
CACGCAG4700.027.765139145
CGATAGG8200.026.70160535-39
TCGTAAG9100.024.85968100-104
CATGGGG95350.024.77241125-29
TACGCAC5450.024.47634350-54
ACATGGG240400.024.44505320-24
Produced by FastQC (version 0.11.5)