FastQCFastQC Report
Sun 19 Mar 2023
SRR4895829_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895829_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919142
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG35080.38166028752902165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCT921550.0143.846011
GCACCTC922100.0143.760222
CACCTCG312100.0143.074143
ACCTCGA90950.0142.564364
ACCTCGG107900.0142.291264
ACCTCAG95400.0142.03734
CACCTCA285600.0141.98223
ACCTCGT111050.0141.95664
CACCTCT307000.0141.246233
ACCTCAT103250.0141.226144
ACCTCTA94950.0140.887654
ACCTCTG100150.0140.629144
CCTCGTA34200.0140.433945
CCTCGAT31050.0140.281165
CTCGATA10850.0140.241556
CCTCGAG29150.0138.786835
CCTCATG41550.0138.154825
CCTCAGT33200.0138.147495
CCTCTGA27100.0138.109045
CTCGTAG11150.0137.761786
Produced by FastQC (version 0.11.5)