FastQCFastQC Report
Sun 19 Mar 2023
SRR4895829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919142
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG123311.3415772535690893No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82030.8924627533068883No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG34620.37665562013268894No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC18450.20073068143986456No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA16000.17407538769852754No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC10760.11706569822725976No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC10350.11260501641748501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT207100.0104.393181
GGTATCA211850.0102.2587668
GTGGTAT210600.0102.246046
AGCAGTG212500.0101.661032
GTATCAA213450.0101.520699
CAGTGGT213400.0100.9604344
AGTGGTA213500.0100.896675
TGGTATC214450.0100.579477
GCAGTGG217900.098.709033
CGTTAGT450.00963797648.334496145
CGGTCTG2900.037.5009145
TGCGGCT4900.029.592546145
CGACCTA3050.025.19595545-49
TACGCAC3100.024.3218450-54
CATGGGG60500.024.11079825-29
AAACCGC8050.023.95579385-89
ATGGGAG34750.023.24178125-29
GCACCTC4154.3655746E-1122.7163332
GAAACCG8500.022.51696485-89
TCCGGCG3150.022.0952935-39
Produced by FastQC (version 0.11.5)