FastQCFastQC Report
Sun 19 Mar 2023
SRR4895828_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895828_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198620
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA19860516.56947155895947No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC425703.5515843219702656No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA331792.7680999816455594No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGCGGATA178311.4876274382206205No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATA178241.4870434332816072No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCATGTACTCTGCGTTGATA176881.4756970516093508No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATA158011.3182660059067928No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGTTGATA136751.1408953630007843No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCATGTACTCTGCGTTGATA111120.9270661260449518No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACGCTGCGTTGATA109760.9157197443726953No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCTGCGTTGATA107740.8988670304183145No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGCTGATA94310.7868215114047821No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCTGCGTTGATA80770.6738582703442292No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACGCTGCGTTGATA78420.6542523902487861No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGTACTCTGCGTTGATA75120.6267207288381639No Hit
AGATGTACTCTGCGTTGATACCACTGCATCCCATGTACTCTGCGTTGATA74950.6253024311291319No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA70470.5879261150322872Illumina Single End PCR Primer 1 (100% over 21bp)
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGTACTCTGCGTTGATA59800.4989070764712753No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGGCGGGTA58240.48589210925898113No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGCGGATA56400.4705411222906342No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCATGTACTCTGCGTTGATAC55060.45936159917238156No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGCGCGGCGGGTA54690.45627471592331176No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACTCTGCGTTGATA53560.44684720762209873No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGTACGCTGCGTTGATA52120.43483339173382723No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGCTGATA50090.4178972485024445No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGATGTACTCTGCGTTGATAC45670.3810215080676111No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCATGTACTCTGCGTTGATA44760.3734294438604395No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGGACGCTGCGTTGATA41920.34973552919190404No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCAGGTACTCTGCGTTGATA36570.3051008659958953No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCACGTACTCTGCGTTGATA36290.30276484623984246No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGCGGGTA34630.2889155862575295No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCATGTACTCTGCGTTGATAC33730.28140695132735977No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGTTGATA26570.2216715889940098No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCAGGTACTCTGCGTTGATA26060.21741669586691362No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGGACTCTGCGTTGATA25590.213495519847825No Hit
AGATGTACTCTGCGTTGAGACCACTGCTTCCCATGTACTCTGCGTTGATA24960.2082394753967062No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGTACGCTGCGTTGATA24900.2077388997346949No Hit
AGATGTACTCTGCCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA23400.19522450818441206No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGTGGATA23360.19489079107640453No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCACGTACTCTGCGTTGATA23190.1934724933673725No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGGACTCTGCGTTGATA22630.1888004538552669No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCGCACGGACGCGGCGCGGATA22120.18454556072817074No Hit
AGATGTACTCTGCGTTGATACCACTGCTACCCATGTACTCTGCGTTGATA21880.18254325808012548No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGGCGTGTA21310.177787789291018No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCTGCGCTGATA21020.17536834025796333No Hit
AGATGTACTCTGCGTTGATAGCACTGCTTCCCATGTACTCTGCGTTGATA20920.1745340474879445No Hit
AGATGTACTCTGCGCTGATACCACTGCTTCCCATGTACTCTGCGTTGATA20650.17228145700889355No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGTGGATA20260.16902771520582002No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCATGGACTCTGCGTTGATA20240.16886085665181624No Hit
AGATGTACTCTGCGTCGATACCACTGCTTCCCATGTACTCTGCGTTGATA20060.1673591296657823No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGGACGCTGCGTTGATA19750.16477282207872387No Hit
AGATGTACTCCGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA19370.1616025095526522No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGCTGATA19230.1604344996746258No Hit
AGATGTACTCTCCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA19200.16018421184362017No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGAACCCGGCGTGGATA19090.15926648979659944No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCACGTACTCTGCGTTGATA18900.15768133353356362No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATGGGCAGAGCGTCGAGAC18860.15734761642555606No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGACGCGCGGCGGGTA18590.15509502594650515No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCAAGGACCCCGCGGTGAAAC18500.15434416245348817No Hit
AGATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGTTGATA17960.14983898149538635No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCAGGTACTCTGCGTTGATAC17330.14458293704426756No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCACGGACTCTGCGTTGATA16710.13941032187015068No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGATGGGCAGAGCGTCGAGAC15740.1313176820009678No Hit
AGATGTACTCTGCGTTGATACCACTGCATCCATGTACTCTGCGTTGATAC15680.1308171063389565No Hit
AGATGTACTCTGCGTTGATACCACTGCATCCCACGTACTCTGCGTTGATA15470.12906509152191686No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAGA15400.12848108658290366No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGTACGCTGCGCTGATA15240.1271462181508735No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGTACGCTGCGTTGATA14920.12447648128681317No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCGCACGGACGCGGCGCGGATA14840.1238090470707981No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGTCGTGTA14470.12072216382172832Illumina Single End PCR Primer 1 (95% over 21bp)
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGCTGATA13840.11546611937060954No Hit
AGATGTACTCTGCGTTGATACCACTGCGGCCCATGTACTCTGCGTTGATA13540.11296324106055296No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACACTGCGTTGATA13340.11129465552051525No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCGCACGGACGCTGCGCTGATA13310.1110443676895096No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCGCACGGACGCTGCGTTGATA12990.10837463082544926No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCATGTACTCTGCGTTGATAC12970.1082077722714455No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACTCTGCGCTGATA12920.10779062588643608No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGATGTACACTGCGTTGATAC12790.10670604528541157No Hit
AGATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATA12590.10503745974537383No Hit
AGATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA12340.1029517278203267No Hit
AGATGTACTCTGCGTTGATACCACTGCCTCCCATGTACTCTGCGTTGATA12090.10086599589527957No Hit
AGATGTACTCTGCGTTGATACCACTGCAACCCATGTACTCTGCGTTGATA12050.10053227878727203No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACACTGCGTTGATAC12050.10053227878727203No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGTA151.2532435E-4144.322088
CTATGAA151.2532435E-4144.322088
CATATGG151.2532435E-4144.322088
CGAGTTA151.2532435E-4144.322088
CTATAAG253.817331E-8144.322088
ACTTCGG100.007191083144.322087
CTGAATG100.007191083144.322088
CGTTAGG202.1810629E-6144.322088
ACGATTG550.0144.322087
CGTAGTT151.2532435E-4144.322088
ACTGTGA500.0144.322087
ACGTCTA100.007191083144.322087
CAGTATG151.2532435E-4144.322088
TACAGTC100.007191083144.322086
CTAGGTG100.007191083144.322088
CTAATGG100.007191083144.322088
CTGTGAG151.2532435E-4144.322088
ACGGTAT800.0144.322087
AGATGTA1199350.0144.231841
GATGTAC1223750.0141.356032
Produced by FastQC (version 0.11.5)