FastQCFastQC Report
Sun 19 Mar 2023
SRR4895827_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895827_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606056
Sequences flagged as poor quality0
Sequence length151
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTC1608200.0144.011832
AGATTCG538950.0143.950413
ACAGATT1610500.0143.806151
GATTCGA153550.0143.359384
AGATTCA486300.0143.300193
GATTCGT198850.0142.971774
GATTCGG184200.0142.912874
GATTCAG165600.0142.811334
GATTCTG179350.0142.27494
AGATTCT555450.0141.8943
GATTCAT171000.0141.885534
ATTCGTG67000.0140.760915
ATTCGGA47650.0140.724325
ATTCGAG49200.0140.687425
GATTCAA152000.0140.504624
ATTCGTA58050.0140.105765
GATTCTA172950.0139.910554
ATTCGAT54350.0139.826725
ATTCGAA48050.0138.65255
ATTCGGT63750.0137.870915
Produced by FastQC (version 0.11.5)