FastQCFastQC Report
Sun 19 Mar 2023
SRR4895827_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895827_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606056
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA173661.0812823463191819No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG118230.7361511678297643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG44800.2789441962173175No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT26840.1671174604123393No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT24910.15510044481637003No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC23730.14775325393386035No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21280.1324984932032258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC18580.11568712423477139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT375200.0118.6579361
GGTATCA375650.0118.3310248
GTGGTAT375250.0117.896856
TGGTATC379200.0116.974687
GTATCAA380900.0116.8604359
AGCAGTG384300.0115.716122
AGTGGTA382800.0115.669865
CAGTGGT383900.0115.443644
GCAGTGG392850.0112.795123
TGCGGCT10150.035.71422145
CGGTCTG5300.030.09428145
GATTCGT2901.2732926E-1130.0046144
CGATAGG3300.026.36407735-39
CATGGGG130500.025.4775725-29
ACATGGG360250.024.84214420-24
CAACGCA377250.024.80810410-14
TCAACGC377700.024.77854710-14
GTACATG375100.024.697520-24
TACATGG373000.024.64217820-24
ATCAACG379000.024.53288510-14
Produced by FastQC (version 0.11.5)