FastQCFastQC Report
Sun 19 Mar 2023
SRR4895826_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895826_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630118
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG38960.6182968904236984No Hit
ACACGACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC7140.11331210979530817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC632650.0143.61211
ACGACCT203750.0143.556383
CACGACC633350.0143.453382
ACGACCG200750.0143.223633
CGACCTG64250.0142.734534
CGACCAT74400.0142.545934
CGACCGT69500.0142.533524
ACGACCA193150.0142.513613
CGACCGA59600.0142.136864
GACCGAT19850.0142.014055
CGACCAG62050.0141.753284
CGACCTT74600.0141.293984
GACCATG30350.0141.105155
CGACCGG69250.0141.069984
CGACCTA65150.0140.984094
GACCTGA17350.0140.868965
GACCGGT19900.0140.570365
GACCGTG21250.0140.461245
GACCGTA20600.0140.343495
GACCGTT21300.0139.793055
Produced by FastQC (version 0.11.5)