FastQCFastQC Report
Sun 19 Mar 2023
SRR4895826_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895826_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630118
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG131302.0837366969361297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65991.0472641632202222No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20390.32359018469556494No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGACAGTGGCTCAA14960.2374158490949314No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT13610.2159912905201883No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCACAGTGGCTCACGCCT13570.21535648878464034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7910.12553204320460612No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTGTAATCTCAGCACTTT7380.11712092020859585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCACGGTGGCTCACG7290.11569261630361297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCACGGTGGCTCAC7200.11426431239863008No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT6860.10886849764647256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT223900.0106.884441
AGTGGTA231000.0103.496885
AGCAGTG231700.0103.15292
TGGTATC232550.0102.931737
GGTATCA233150.0102.915578
CAGTGGT232550.0102.838224
GTGGTAT232550.0102.8070456
GTATCAA235050.0102.215169
GCAGTGG235600.0101.322293
TGCGGCT2700.059.07673145
CGAGCCG1101.6734703E-952.729637145
TAATGAT7000.031.072824145
CGCTGTT2351.2605597E-930.85245145
AGAGAGT7300.029.795858145
AGGGGTA1555.5015065E-528.0613215
GGTACGA507.844574E-826.099125-29
CATGGGG44650.025.62173725-29
ATGGGGG19200.024.69446225-29
ATGGGAT34950.023.85468725-29
ATGGGAG32750.022.97783325-29
Produced by FastQC (version 0.11.5)