FastQCFastQC Report
Sun 19 Mar 2023
SRR4895825_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895825_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237291
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT26661.1235150089973913No Hit
AATGTTGCGTTGATACCACTGCTTCCCATGTACTCGGCGTTGATACCACT3780.15929807704464138No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG151.2561704E-4144.201288
CAGATGC151.2561704E-4144.201288
TGCGATT2400.0144.201286
TGCGACT306.730261E-10144.201286
CTAGAGA202.1865144E-6144.201268
CGTATAA100.007205492144.201268
GCGAATG750.0144.201267
CGATTGA351.2732926E-11144.201268
CGATTAG253.82679E-8144.201268
GCGATTG800.0144.201267
GCGATTA650.0144.201267
CGGTAGT500.0144.201268
TGCGAGT2500.0144.201256
ATGTTGC237900.0143.807282
AATGTTG238350.0143.535771
TGTTGCA67800.0143.350523
TGTTGCG81850.0143.14423
GTTGCGA21200.0142.840884
GTTGCAT24500.0142.435534
TTGCGAA6500.0141.982775
Produced by FastQC (version 0.11.5)