FastQCFastQC Report
Sun 19 Mar 2023
SRR4895824_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895824_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339308
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTC1340350.0144.095282
AATCCGT1340900.0144.036161
TCCGTCA400550.0143.84823
TCCGTCG456350.0143.560443
CCGTCGA133600.0143.236774
CCGTCAG135700.0143.09244
CCGTCTA136150.0142.884264
TCCGTCT446800.0142.820373
CCGTCAT150150.0142.527514
CGTCGTA48650.0141.98515
CGTCAGT47650.0141.938455
CCGTCGT169700.0141.913824
CCGTCAA116150.0141.849264
CGTCAGG44700.0141.788645
CGTCTAG43600.0141.396845
CCGTCTG137050.0141.262014
CGTCGAA39800.0141.128425
CGTCATA41750.0141.099565
CGTCAGA36400.0140.840745
CGTCAAG40200.0140.800345
Produced by FastQC (version 0.11.5)