FastQCFastQC Report
Sun 19 Mar 2023
SRR4895824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339308
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA136311.0177643977337552No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG76820.5735797889656449No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG20450.15269079255854517No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT18410.13745904601480766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC16700.12469125847079238No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT15020.11214746719947913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC14950.1116248092298411No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC14510.10833953056354476No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13800.10303828544293023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT268950.0117.580036
GGTATCA270500.0117.49598
AAGCAGT271350.0117.457221
TGGTATC272300.0116.293237
AGTGGTA272800.0115.960195
GTATCAA274800.0115.7628869
CAGTGGT276150.0114.632244
AGCAGTG278650.0114.015852
GCAGTGG282050.0112.260043
CGTCGAA554.0606865E-665.913665
TCCGTCG1350.064.4489063
CCGTCGA951.92046E-645.792654
GGCTATC6250.032.4774145
AATCCGT6100.032.0911871
ATCCGTC6900.030.4719892
GCTATCC4900.028.109993145
TGCGGCT10100.027.275045145
TACGCAC6000.026.58140450-54
CGACCTA6100.026.14642545-49
CGATAGG4900.025.4507435-39
Produced by FastQC (version 0.11.5)